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Volume 39 Number 3 Volume 39 Number 4 Volume 39 Number 5

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Research articles

ScienceAsia 39(2013): 384-391 |doi: 10.2306/scienceasia1513-1874.2013.39.384

A feasibility study of oil content and fatty acid composition of seed powder and seed oil of Pongamia pinnata by near infrared spectroscopy

Chutarat Khamchuma, Vittaya Punsuvona,b,c,*, Sumaporn Kasemsumrand, Nattaporn Suttiwijitpukdeed

ABSTRACT:     Near infrared reflectance spectroscopy (NIRS) coupled with a geometric study was used to estimate the oil content and fatty acid composition of Pongamia pinnata seed powder and seed oil. Sixty seed powders and seed oils from various sources of P. pinnata in southern Thailand were scanned by NIRS. All samples were analysed for oil content by hexane extraction and fatty acid composition by gas chromatography. Calibration equations were developed for oil content and individual fatty acids (palmitic acid C16:0, stearic acid C18:0, oleic acid C18:1, linoleic acid C18:2, and behenic acid C22:0). The results showed that NIRS was a reliable and accurate technique to estimate oil content from seed powder, as well as the concentrations of oleic, behenic, and linoleic acid in seed oil. Screening by NIRS of P. pinnata seed powders and seed oils represents a rapid, simple, and cost-effective alternative method that may be of great utility for analysing a large number of seed samples for tree breeding and plantations for the biofuel industry.

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a Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok 10900 Thailand
b Centre of Excellence-Oil Palm, Kasetsart University, Bangkok 10900 Thailand
c Centre of Advanced Studies in Tropical Natural Resource, NRU-KU, Kasetsart University, Bangkok 10900 Thailand
d Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok 10900 Thailand

* Corresponding author, E-mail: fscivit@ku.ac.th

Received 6 Nov 2012, Accepted 17 May 2013