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Volume 38 Number 4 Volume 39 Number 1 Volume 39 Number 2

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Research articles

ScienceAsia 39 (2013): 26-35 |doi: 10.2306/scienceasia1513-1874.2013.39.026

Characterization of xylose-utilizing yeasts isolated from herbivore faeces in Thailand

Wanlapa Lorliama, Ancharida Akaracharanyab, Sasitorn Jindamorakotc, Surisa Suwannarangseed, Somboon Tanasupawata,*

ABSTRACT:     A total of 39 xylose-utilizing yeast strains were isolated from herbivore faeces in Thailand. They were identified as Candida tropicalis (32 isolates), Candida albicans (1 isolate), Pichia terricola (1 isolate), Trichosporon mycotoxinivorans (2 isolates), Sporopachydermia lactativora (2 isolates) and Zygoascus meyerae (1 isolate) based on their morphological, cultural, physiological and biochemical characteristics including the sequence analysis of the D1/D2 region of the large-subunit ribosomal DNA. Thirty seven isolates could ferment xylose to ethanol. Zygoascus meyerae E23 isolated from elephant faeces produced the highest ethanol concentration (3.61 g/l after 72 h). C. tropicalis A26 isolated from cow faeces produced the highest xylitol concentration (43.79 g/l) which corresponded to 0.71 g xylitol/g xylose after 24 h. C. tropicalis A26 xylose reductase showed 98.4% identity and 99.0% similarity to C. tropicalis (ABX60132C) xylose reductase, and showed the tetra-amino acid motif (Ile-Pro-Lys-Ser) which is conserved among NADPH-dependent xylose reductase.

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a Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330 Thailand
b Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330 Thailand
c BIOTEC Culture Collection, Bioresources Technology Unit, National Centre for Genetic Engineering and Biotechnology (BIOTEC), Pathum Thani 12120 Thailand
d Enzyme Technology Laboratory, Bioresources Technology Unit, National Centre for Genetic Engineering and Biotechnology (BIOTEC), Thailand Science Park, Pathum Thani 12120 Thailand

* Corresponding author, E-mail: Somboon.T@chula.ac.th

Received 18 May 2012, Accepted 26 Nov 2012