ScienceAsia 38(2012): 30-35 |doi:
Antibiotics resistance and RAPD-PCR typing of multidrug resistant MRSA isolated from bovine mastitis cases in Thailand
Manakant Intrakamhaenga, Tanaya Komutarinb,*
ABSTRACT: Methicillin resistant Staphylococcus aureus (MRSA) strains are a global health concern in both human and veterinary medicine. In addition to β-lactam resistance, MRSA can carry resistance to other commonly used antibiotics. The present study sought to determine the antibiotic susceptibility profile and genetic relatedness of strains emerging in Thailand. A total of 229 isolates of S. aureus obtained from 598 mastitis cases were investigated for their susceptibility to several antibiotics. Among 229 isolates of S. aureus, 27 were found to be methicillin resistant and multidrug resistant. Multidrug resistant MRSA infection was detected in 4 provinces including Lopburi, Udon Thani, Khon Kaen and Saraburi. Multidrug resistant strains exhibited several antibiogram patterns (antibiotypes I to XII). The most frequent pattern was antibiotype VII, i.e., resistance to most commonly used antibiotics. Based on random amplification of polymorphic DNA (RAPD) technique and a combined amplicon-profile obtained with three primers, all isolates were grouped according to genetic similarity. Five different RAPD patterns (types I to V) were identified with 90% genetic similarity. The close genetic relatedness within RAPD type I was interesting because most RAPD type I isolates had antibiogram pattern III or VII. In addition, some isolates from different regions were identical in both antibiogram pattern and RAPD type. This revealed that some of the antibiotic-resistant isolates in our study were epidemic strains.
||Department of Biology, Faculty of Science, Maha Sarakham University, Mahasarakham, Thailand
||Faculty of Medicine, Maha Sarakham University, Mahasarakham, Thailand
* Corresponding author, E-mail: email@example.com
Received 5 Oct 2011, Accepted 11 Mar 2012